Panherpes PCR with Degenerate Primers

The method is based on the fact that herpesvirus genomes contain highly conserved genes, which are present in all because they code for proteins essential for viral growth. One of the most conserved is the herpesvirus DNA polymerase (DPOL) gene. Investigators aligned the DPOL genes of all herpesviruses for which sequence data were available, in order to identify short blocks of greatest amino acid identity that encode domains essential for DPOL function. This procedure allowed the design of...