Figure 123

Uptake curve and Michaelis-Menten double-reciprocal plot. On the left is shown the uptake activity in Hurler cells as a function of medium concentration of iduronidase. Note that the activity in the cells rises and the reaches a plateau when the receptor-mediated uptake reaches saturation. On the right is a double-reciprocal plot of the uptake data that shows a linear relationship. From the intercept, Vmax can be calculated and from the Vmax and slope, the apparent KM can be determined, shown here in enzyme units/mL.

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