Like type II (sometimes three structural genes)
Cut at fixed distance from asymmetric recognition sites
5'-GGATG (N9) 3'-CCTAC (N13)
Two structural genes; Afunctional; multisubunit
Cut DNA at defined distances, on both sides of the recognition sequence
Several subunits and genes, no modification enzymes
Cut DNA with modified C residues at different positions between the two RmC sites
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