Iis

Like type II (sometimes three structural genes)

Mg2+

Cut at fixed distance from asymmetric recognition sites

Fofcl

5'-GGATG (N9) 3'-CCTAC (N13)

Scgl-like

Two structural genes; Afunctional; multisubunit

Mg2+, AdoMet

Cut DNA at defined distances, on both sides of the recognition sequence

Bcgl

(N10)CGA(N6)TGC(N12) (N12)GCT(N6)ACG(N10)

McrBC

Several subunits and genes, no modification enzymes

Mg2+, GTP

Cut DNA with modified C residues at different positions between the two RmC sites

McrBC

5'-RmC(N4o_2ooo)RmC

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