Chp

1291 (422)

908(293) -

1681 (524) -

Phosphorylation of P protein by CKII is critical for RNA synthesis by the RNA polymerase complex (L-P). The region (aa 55-70) of P containing the site(s) of phosphorylation of CHP, Piry (?), VSVNJ and VSV|nd are shown below.

Piry

1338(427)

999(328) 745(230)

1683(529) -

55 70 CHP: DECEEED*SEEDDDNLP Piry: GSMLKESDESESVDDD VSVimj: EEES*SD*SDTDYNAEHL VSV,nd: QAADD*SD*TE*SEPEIED

Nucleotide lengths of mRNAs excluding poly(A) tail are shown, with the protein size in amino acids in parentheses. The asterisks indicate CKII phosphorylation sites. In the case of Piry virus, the phosphorylation site(s) remains unknown. However, three Ser residues, Ser 72, Ser 89 and Ser 94 in an acidic region (not shown) conform to the consensus CKII recognition motif.

Nucleotide lengths of mRNAs excluding poly(A) tail are shown, with the protein size in amino acids in parentheses. The asterisks indicate CKII phosphorylation sites. In the case of Piry virus, the phosphorylation site(s) remains unknown. However, three Ser residues, Ser 72, Ser 89 and Ser 94 in an acidic region (not shown) conform to the consensus CKII recognition motif.

are highly dissimilar. However, the hydropathicity patterns of these proteins are similar, indicating that the overall secondary structure of P proteins may be conserved. Gene sequences of ISF virus are not known to date.

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