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aHomology of primers to published virus sequences (GenBank); X/Y/Z score: X, number of published sequences overlapping the primer-binding site (the more the better); Y, sequences containing > 5 mismatches (the lesser the better); Z, sequences containing > 2 mismatches within 5 bases from the 3'-end of the primer or a mismatch at the ultimate 3'-base of the primer (the lesser the better). Abbreviations: g/ag, genomic/antigenomic primer; p, probe; SB, Southern blot; FAM, 6-carboxyfluorescein; TX, 6-carboxytetramethylrodamine.

West African strain sequences only.

aHomology of primers to published virus sequences (GenBank); X/Y/Z score: X, number of published sequences overlapping the primer-binding site (the more the better); Y, sequences containing > 5 mismatches (the lesser the better); Z, sequences containing > 2 mismatches within 5 bases from the 3'-end of the primer or a mismatch at the ultimate 3'-base of the primer (the lesser the better). Abbreviations: g/ag, genomic/antigenomic primer; p, probe; SB, Southern blot; FAM, 6-carboxyfluorescein; TX, 6-carboxytetramethylrodamine.

West African strain sequences only.

fever, the diagnosis was established by PCR before the development of specific IgM.

Apart from yellow fever and dengue virus, rare flaviviruses such as Kyasanur Forest virus or Alkhurma virus can cause a hemorrhagic fever.[41,42] Use of universal flavivirus PCRs is appropriate when these infections are suspected and typical VHF agents have been ruled out.

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