Many laboratories still use IEF to determine protein type. This requires considerable expertise for the interpretation of banding patterns and relies on sample stability for reliable interpretation. The resolution can be improved with ultrathin polyacrylamide gels and sensitivity can be improved by immunoblotting. Many laboratories use DNA-based methods involving polymer-ase chain reaction and either allele-specific amplification, for example, or the introduction of a restriction enzyme site at the location of the Z variant.[21,22] The DNA-based assays are designed to look for the common deficiency variants, S and Z, and will not detect rare variants. This is
Was this article helpful?