IEF and Genetic Testing

Many laboratories still use IEF to determine protein type.[19] This requires considerable expertise for the interpretation of banding patterns and relies on sample stability for reliable interpretation. The resolution can be improved with ultrathin polyacrylamide gels[20] and sensitivity can be improved by immunoblotting. Many laboratories use DNA-based methods involving polymer-ase chain reaction and either allele-specific amplification, for example, or the introduction of a restriction enzyme site at the location of the Z variant.[21,22] The DNA-based assays are designed to look for the common deficiency variants, S and Z, and will not detect rare variants. This is

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