Factors Affecting The Evolution Of Resistance

As an evolutionary trait, insecticide resistance is unusual in that we can identify the main selection pressure with ease, but the rate at which resistance develops is governed by numerous biotic and abiotic factors. These include the genetics and ecology of the pests and their resistance mechanisms, and the operational factors that relate to the chemical itself and to its application. To manage resistance effectively, an assessment of genetic, ecological, and operational risk is required. Although this can be done empirically on a species-by-species basis, one of the great challenges of the future is to understand why some species seem to have a greater tendency to become resistant than others.

Genetic Influences

To predict how quickly resistance will become established, it is necessary to understand how resistant alleles affect the survival of phenotypes in the field. For example, the dominance of resistance genes exerts a major influence on selection rates. In laboratory bioassays evaluating the relative survival of susceptible homozygotes (SS), heterozygotes (RS), and resistance homozygotes (RR) over several insecticide concentrations, RS individuals usually respond in an intermediate manner. In the field, however, dominance is dependent on the concentration of insecticide applied and its uniformity over space and time. Even when the initial concentration is sufficient to kill RS individuals (rendering resistance effectively recessive), upon weathering or decay of residues, this genotype may later show increased survival, with resistance becoming functionally dominant in expression. When resistance genes are still rare, hence mainly present in heterozygous condition, this sequence can have a profound effect in accelerating the selection of resistance genes to economically damaging frequencies.

The diverse mating systems of insects also influence the rate at which resistance evolves. Although most research has focused on outcrossing diploid species (typified by members of the Lepidoptera, Coleoptera, and Diptera), systems based on haplodiploidy and parthenogenesis also occur among key agricultural pests. In haplodiploid systems, males are usually produced uniparentally from unfertilized, haploid eggs, and females are produced biparentally from fertilized, diploid eggs. The primary consequence of this arrangement (exemplified by whiteflies, spider mites, and phytophagous thrips) is that resistance genes are exposed to selection from the outset in the hemizygous males, irrespective of intrinsic dominance or recessiveness. Whether a resistance gene is dominant, semidominant, or recessive, resistance can develop at a similar rate.

Most species of aphid undergo periods of parthenogenesis (in which eggs develop and give rise to live offspring in the absence of a paternal genetic contribution) promoting the selection of clones with the highest levels of resistance and/or the most damaging combination of resistance mechanisms. In fully anholocyclic (asexual) populations, such as those of M. persicae in northern Europe, the influence of parthenogenesis has led to strong and persistent associations between resistance mechanisms within clonal lineages.

Ecological Influences

Fecundity and generation times have a huge bearing on the evolution of resistance in a population. The greater the number of individuals, and the faster they reproduce and attain maturity, the higher the likelihood that a favorable mutation will occur, and be maintained in the population. Faster growth and higher population numbers will also have an effect on the size of a pest population, and therefore the need for insecticide treatment.

The dispersal capabilities of pests can also act as primary determinants of resistance development. Movement of pests between untreated and treated parts of their range may delay the evolution of resistance because of the diluting effect of susceptible immigrants. Conversely, large-scale movement can also accelerate the spread of resistance by transferring resistance alleles between localities. A good example relates to the two major bollworm species (Lepidoptera: Noctuidae) attacking cotton in Australia. Only the cotton bollworm Helicoverpa armigera, has developed strong resistance. H. punctigera, despite being an equally important cotton pest, has remained susceptible to all insecticide classes. The most likely explanation is that H. punctigera occurs in greater abundance on a larger range of unsprayed hosts than H. armigera, thereby maintaining a large pool of unselected, susceptible individuals, which dilute resistant mutations arising on treated crops.

Operational Influences

Operational factors are at human discretion and can be manipulated to influence selection rates. Factors exerting a major influence in this respect include the rate, method, and frequency of applications, their biological persistence, and whether insecticides are used singly or as mixtures of active ingredients.

Equating operational factors with selection is often difficult, since without detailed knowledge of the mechanisms present it is impossible to test many of the assumptions on which genetic models of resistance are based. If resistance alleles are present, the only entirely nonselecting insecticide doses will be ones sufficiently high to overpower all individuals, regardless of their genetic composition, or ones so low that they kill no insects at all. The latter is obviously a trivial option. Prospects of achieving the former depend critically on the potency and dominance of resistance genes present. A pragmatic solution to this dilemma is to set application doses as far above the tolerance range of homozygous, susceptible individuals as economic and environmental constraints permit, in the hope that any heterozygotes that do arise will be effectively controlled. However, this approach will obviously be ineffective if resistance turns out to be more common than suspected (resulting in the presence of homozygous resistant individuals) or if resistance alleles exhibit an unexpectedly high degree of dominance (and heterozygotes are therefore phenotypically resistant). Unless a high proportion of insects escape exposure altogether, the consequence could then be very rapid and effective selection for homozygous resistant populations.

In practice, concerns about optimizing dose rates to avoid resistance are secondary to those related to the application process itself. Delivery systems and/or habitats promoting uneven or inadequate coverage will generally be more prone to select for resistance, because, under these circumstances, pests are likely to encounter suboptimal doses of toxins that will permit survival of heterozygous individuals.

The timing of insecticide applications relative to the life cycle of a pest can also be an important determinant of resistance. A good example of this is found in the selection of pyrethroid resistance in H. armigera in Australia. On cotton foliage freshly treated with the recommended field dose, pyrethroids killed larvae up to 3 to 4 days old irrespective of whether they were resistant by laboratory criteria. Since the sensitivity to pyrethroids of larvae of all genotypes was found to decline with increasing larval size, the greatest discrimination between susceptible and resistant phenotypes occurred when larvae achieved a threshold age. Targeting of insecticides against newly hatched larvae, as is generally advocated for bollworm control, not only increases the likelihood of contacting larvae at the most exposed stage in their development but also offers the greatest prospect of retarding resistance by overpowering its expression. It may also have the effect of reducing genetic variation and therefore the potential number of resistant mutations. Indeed, it is also possible to impose genetic "bottlenecks" by applying pesticides when populations are already low (e.g, when they are overwintering). Although such a tactic might be beneficial where populations are fully susceptible, if resistant mutations are already present, it might act to increase their frequency.

In theory, the application of two or more unrelated chemicals as insecticide mixtures offers substantial benefits for delaying the selection of resistance. The underlying principle is one of "redundant killing," whereby any individuals already resistant to one insecticide are killed by simultaneous exposure to another, and vice versa. However, achieving this objective requires that each type of resistance be rare and that both ingredients persist throughout the effective life of an application. Otherwise, one compound will exert greater selection pressure than the other, and the advantage of applying a mixture will be lost.

Fitness of Resistant Individuals

In the absence of insecticidal selection pressure, resistance genes can impose fitness costs on their carriers. Sometimes these costs are quite subtle and difficult to determine. In M. persicae, resistant individuals are less inclined to move from senescing to younger leaves and are therefore more vulnerable to isolation and starvation after leaf abscission. These costs appear to contribute to a decline in the frequency of resistant insects between cropping seasons.

Bee Keeping

Bee Keeping

Make money with honey How to be a Beekeeper. Beekeeping can be a fascinating hobby or you can turn it into a lucrative business. The choice is yours. You need to know some basics to help you get started. The equipment needed to be a beekeeper. Where can you find the equipment you need? The best location for the hives. You can't just put bees in any spot. What needs to be considered when picking the location for your bees?

Get My Free Ebook

Post a comment