Interaction of the Microbiome with the Innate Immune Response in Chronic Wounds

Segre Abstract Microbes colonizing and or infecting chronic wounds undoubtedly play a major and interactive role in impaired healing, especially in amplifying and perpetuating the host innate immune response. The development of molecular techniques to identify and quantify microbial organisms has revolutionized our view of the microbial world. These less-biased, high throughput methods greatly enable investigations regarding host-microbe interactions in the...

Conditioning for Transplantation Activates Complement Cascade CC in BM

We noticed that similarly as during mobilization, conditioning for transplantation by lethal irradiation activates CC in BM. The activation of CC during conditioning for transplantation was confirmed by ELISA to detect C3a and C5a cleavage fragments in plasma(Lee et al. 2010) and histochemical detection of MAC in BM tissue (Ratajczak et al. 2010). The importance of CC in stem cell homing engraftment is supported by fact that C3- and C5-deficient mice show delayed recovery of hematopoietic...

The Pentraxin Superfamily

Pentraxins are characterized by the presence in their carboxy-terminal region of a 200 amino acid long domain containing a highly conserved motif of primary sequence known as pentraxin signature (HxCxS TWxS, where x is any amino acid). The prototype of the family is CRP, a molecule identified in the 1930s for its ability to bind in a calcium-dependent fashion the C-polysaccharide of Streptococcus pneumoniae (Tillet and Francis 1930). Human SAP, identified as a closely related protein (i.e. with...

PTX3 in Pathogen Recognition and Inflammation

PTX3 interacts with a number of different pathogens including selected fungi, virus and bacteria (Fig. 1). A specific binding has been observed to Aspergillus fumigatus conidia, zymosan, Paracoccidioides brasiliensis, selected Gram positive and Gram negative bacteria, including Staphyloccocus aureus, Pseudomonas aeruginosa, Salmonella typhimurium, Streptococcus pneumoniae and Neisseria menin-gitides and with human and murine cytomegalovirus (CMV) and H3N2 influenza virus (Garlanda et al. 2002...

List of Contributors

Thomas Areschoug Department of Laboratory Medicine, Division of Medical Microbiology, Lund University, S lvegatan 23, 22362 Lund, Sweden e-mail Thomas.Areschoug med.lu.se Jennie B ck Dept of Immunology, Genetics and Pathology IGP , Rudbeck Laboratory C5 3, Uppsala University, SE-751 85 Uppsala, Sweden e-mail jennie.back igp.uu.se O. L. Brekke Department of Laboratory Medicine, Research Laboratory, Nordland Hospital, Bod0, Norway Institute of Medical Biology, University of Troms0, Troms0, Norway...