Immortal Ebook

Discover The Secret Of Immotality

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Author: Chris George
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Advantages And Principles Of The Sage Methodology

Major advantages of the SAGE method are (i) that the information generated is digital in format (ii) that the data obtained can be directly compared with data generated from any other laboratory or with data available in public databases and (iii) the information generated is virtually immortal, and it has the advantage of being constantly updated and subject to reinterpretation, since the more we learn on the identification of new transcripts, the more complete and accurate the SAGE datasets become.

Flow Analysis and Sorting of Plant Chromosomes

Basic Protocol 1 provides a procedure for accumulation of cells in metaphase. Originally developed for the field bean (Vicia faba), this procedure is also suitable for other large-seeded legumes such as the garden pea (Pisum sativum). A modification of the procedure that is suitable for cereals such as barley (Hordeum vulgare) and rye (Secale cereale) is given in Alternate Protocol 1. Both protocols were developed for use with roots of young seedlings. The modification of Basic Protocol 1 for the use with immortal root (hairy root) cultures is described in Alternate Protocol 2. The extent of cell-cycle synchrony may be monitored according to Support Protocol 1. Preparation of chromosome suspensions from synchronized root tips is described in Basic Protocol 2.

Real Time Detection and Quantification of Telomerase Activity Utilizing Energy Transfer Primers

Telomeres, the specific structures found at the end of eukaryotic chromosomes are essential for stabilization of chromosome ends (1,2). Chromosomes lacking telomeres undergo fusion, rearrangement, and translocation (1). In somatic cells, telomeres are progressively shortened during each cycle of cell division owing to the inability of the DNA polymerase complex to replicate the very 5'-end of the lagging strand of DNA (3,4). Telomerase, a ribonucleopro-tein reverse transcriptase (5,6), adds telomeric repeats onto the 3'-end of telomeres, thereby compensating for the gradual loss of telomeres (1). Telomerase activity is repressed in most somatic tissues but is found in germline cells, stem cells, cancer cells, and immortal cells (7,8), which suggests a close association between telomerase expression and immortalization cellular proliferation capacity. In fact, de novo expression of the telomerase activity extends the lifespan of normal human cells (9). These findings have made

Virus Propagation in Cell Culture

Cell lines derived from cancer tissues, capable of indefinite growth in culture, and strains of diploid cells prepared from human or animal tissues (in particular from embryos), capable of growing in culture up to 100 cell generations, contain cells of a specific type and allow the propagation of viruses under defined conditions. The choice of the most suitable cell line to propagate a virus will depend mainly on the host range and tissue specificity of the virus investigated. Continuous cell lines are useful for the propagation of viruses such as adenoviruses and rhinoviruses. Insect viruses (e.g. baculoviruses) can be propagated in vitro in immortal insect cells (e.g. SF cells, derived from Spodoptera frugiperda pupal ovarian tissue). Other viruses, like herpesviruses or enteroviruses, may preferentially be propagated in diploid cell strains of finite life.

Asymmetric Mitoses and the Stem Line Mutation Rate

Cairns (1975) emphasized that in a stem-transit architecture, only the stem lineage survives over time. Thus, only those mutations in the immortal stem lineage remain in the tissue. Cairns argued that organisms may use various mechanisms to reduce the mutation rate in the stem lineage. Figure 12.10 Cairns' (1975) hypothesis of asymmetric DNA segregation in stem cell divisions. (a) Immortal stranding, in which the stem lineage along the bottom always receives the older strand of the DNA duplex in each round of cell division. (b) Segregation of the newer DNA strand to the stem cell lineage in each round of cell division. Random segregation would follow a stochastic process between these two patterns. See text for full discussion. Figure 12.10 Cairns' (1975) hypothesis of asymmetric DNA segregation in stem cell divisions. (a) Immortal stranding, in which the stem lineage along the bottom always receives the older strand of the DNA duplex in each round of cell division. (b) Segregation of...

Human Papilloma Virus and EGFlike GFs and Receptor Expression

Approximately 66 of cervical carcinomas and 100 of vulvar condylomas that are the result of HPV-infection express high levels of TGF-a mRNA (325). Because HPV-16 and HPV-18 are associated with approx 60-90 of cervical intraepithelial neoplasias, and since these viruses have a major transforming potential, these data suggest that overexpression of TGF-a may be associated with the early stages of disease after HPV infection. In this context, the E6 and E7 genes are involved in the immortalization and transforming activities of HPV, and the effects of these genes are accentuated by the product of the E5 gene (334). The E5 gene encodes for a small membrane-associated protein that can inhibit the downregulation and degradation of the EGFR in response to ligand binding. In addition, E5 can induce hyperphosphorylation of the EGFR in a ligand-independent fashion, and can accelerate receptor recycling at the cell surface, thereby hypersensitizing infected cells to the effects of EGF-related...

Expression Patterns and Biological Effects of EGFRelated GFs

GFs have been implicated as agents that stimulate proliferation and enhance the possibility of malignant transformation in cervical cells (351). In proliferation assays, EGF can enhance CC cell growth in a dose-dependent manner (352,353). EGF may be stimulating proliferation by an EGF mitogenic pathway, and by indirectly reducing the levels of inhibitory insulin-like GF binding proteins. It has also been demonstrated that the autocrine production of IGF-II and the overexpression of IGF-IR, are important in controling the proliferation of CC cells, and that autocrine IGF-II production may also participate in the mitogenic signaling of EGF in these cells (354). This might be an important mechanism, since Tonkin et al. (355) did not find evidence of amplification of the EGFR gene in four CC cell lines, using Southern blot analysis. Additionally, infection with multiple sexually transmitted pathogens has been associated with inflammation of the cervix and an increased risk of CC in women...

Role of Oxidative Stress in Aging of the Yeast Saccharomyces cerevisiae

The idea of using the yeast Saccharomyces cerevisiae as a model organism in the studies of aging may appear weird. Yeast as all microorganisms seems to be immortal (unless really badly treated) and there is no doubt that inoculating a yeast colony or yeast mass bought in a grocery shop will always yield a viable yeast culture. However, yeast does age at least in two respects, which are usually referred to as replicative and chronological aging, respectively.

Serious Illness And Other Unanticipated Absences

Both therapists and clients tend to find comforting the myth that the therapist is immortal and invulnerable (Pope, Sonne, & Greene, 2006). Therapists may enjoy the feeling of strength and of being a perfect care-giver that such a fantasy, which sometimes occurs on an unconscious level, provides. Clients may soothe themselves (and avoid confronting some personal issues) with the fantasy that they are being cared for by an omnipotent, immortal parental figure. Although we have not completed our careful study of every therapist who has ever lived, our preliminary results suggests that there has yet to appear a therapist who is immortal and invulnerable. For all of us who are mortal and vulnerable, it is important to prepare for those unexpected times when we are suddenly unavailable to our clients (see Chapter Six).

Simple Counting Mechanism

For the first half of the twentieth century it was believed that cells cultured in laboratory glassware could replicate indefinitely if the correct nutrient media and other conditions of growth could be found. Repeated initial failure at culturing indefinitely replicating cells was followed by success in the late 1940s, when the immortal L929 cancer cell population was developed from mouse tissue. Later, other immortal cell populations were found, including the first human cell line, HeLa, derived from a human cervical carcinoma.

Creating a Professional Will

Nless a therapist is invulnerable and immortal, it is a good idea to prepare a professional will. One focus of this book is the therapist as human. Part of our humanity is our mortality and our vulnerability. Unpleasant as it is to think about, all of us are vulnerable to the unexpected. At any time a drunk driver, a stroke, a mugging, a heart attack, a fire, a plane crash, and countless other misfortunes may bring our life to a sudden and unexpected end. It is an ethic of both personal and professional responsibility to take our mortality and vulnerability into account in our planning.

Banana

In fertility cults in West Africa, the banana is an obvious male symbol, with its phallic-shaped fruit. It is often used in the symbolism in combination with the terminal bud, which, with its oval form, stands for the female reproductive organs (Talbot). In Uttar Pradesh, the image of the goddess Nanda Devi is carved from the trunk of a banana tree, and the fruit is a symbol of fecundity - a newly married bride is given the fruit. If a baby is born prematurely, the new-born child is made to sleep each day on a fresh banana leaf (Upadhyaya). Another kind of symbolism is used by some Negrito groups in Malaysia, who use the banana plant in stories to explain man's mortality. When the deity gave to one of the superhumans some water life-soul to give to the humans they had made, it was inadvertently lost. So the superhuman borrowed some from a banana plant. This was wind life-soul that he then gave to the inert bodies. Wind life-soul is a short life-soul, whereas what had been lost (the...

Human Origins

Would have made man immortal, whereas the one that the human beings eventually received was merely borrowed and thus provides only temporary life. Some say that not only the life-soul but also the heart and blood were borrowed from a banana plant, and this is supposed to account for the resemblance, in colour and viscosity, between coagulated banana plant sap, which dries to a dark brown colour, and dried human blood (Endicott). In West Africa, men and women, so it is said, descended to earth from the branches of a huge mythical IROKO (Chlorophora excelsa) tree (Parrinder).

Hildrop John

John Hildrop ( -1756) was an English cleric and author of the benchmark work Free Thoughts upon the Brute-Creation (1742), in which he critiqued the work of a French Jesuit, Father Bougeant, for his view that animals have no reason, understanding, moral status, or immortal soul. Originally in the form of two letters to a lady, his work directly confronts the major elements of traditional Aristotelian Thomistic thought and is one of the earliest and most sophisticated zoophile books ever published. He also critiqued John Locke's materialist view of animal rationality in Locke's Essay Concerning Human Understanding (1690) Why does Locke take so much pains to persuade himself and us, that Rationality in Brutes must proceed from a quite different cause, from what it does in ourselves What is he afraid of '' (15). Hildrop was rector of Wath in Yorkshire and chaplain to the earl of Ailesbury and Elgin. After Thomas Tryon, who introduced the word rights in the nonhuman context, his work may...

Mustard Tree

(Myrtus communis) An evergreen, used by the Greeks as a symbol both of love and immortality. Because they regarded their great men as immortal, both Greeks and Romans put myrtle wreaths on the heads of their successful poets and playwrights (Moldenke). In the Scriptures, myrtle is referred to chiefly as a symbol of divine generosity, and throughout the Bible it is emblematic of peace and joy, and also of justice (Moldenke). But it is the love symbolism that is the most important. It was held to have the power of

Orpine

In Iowa, they say that a live-forever plant kept in the room will prevent cancer (Stout). The shape of the root tubers signal orpine with virtue against the King's Evil (Grigson. 1955), or scrofula, and the plant was recommended for fevers, sterility in women and too profuse menstruation (Fluck). Coles advised that the leaves, bruised and applied., to the throat cureth the Quinsy .

Animal Theology

Animal theology relates Christian thinking to contemporary debates about the status and rights of the nonhuman animals (see ANIMAL RIGHTS). It seeks to address and redress the failure of historical theology to take seriously alternative insights that lie largely silent within the Christian tradition (see RELIGION AND ANIMALS, Christianity). Systematic theology has largely proceeded on the basis of the virtual nonexistence of animals. Historically, animals have been the outcasts of theology, defined as beings with no mind, reason, immortal soul, or moral status (see MORAL STANDING OF ANIMALS). Basic questions about their status and significance have simply not been addressed. The question raised by animal theology is whether Christian doctrine is necessarily speciesist (see SPECIESISM) and whether it can incorporate animal-centered concerns into mainstream thinking. Modern theologians argue variously that even conservative theological understandings can be enhanced and deepened by the...

Christianity

Second, and allied to instrumentalism, there has been a consistent hu-manocentricity (see ANTHROPOCENTRISM) that has effectively defined animals out of the moral picture. This has been achieved largely through the emphasis upon certain perceived differences between humans and animals. Animals are judged as beings with no reason or immortal soul and

Madonna Lily

(Lilium candidum) Probably the oldest domesticated flower, known to have been grown in Crete from about 3000BC (Woodcock & Stearn). An old name for this lily is Juno's Rose. The legend is that Jupiter, to make his infant son Hercules immortal, put him to the breast of Juno. The drops of milk that fell to the ground became white lilies, while those that went into the sky became the Milky Way. But this lily is better known, as the common implies, for its association with the Virgin Mary. The Buckinghamshire name Lady Lily is relevant in fact any plant name referring to 'Lady' or 'Lady's' must always show there was a connection with the Virgin. But it is this lily that is the Virgin's plant par excellence, and her emblem, always shown in pictures of the Annunciation. Indeed, the lily of sacred art is always the Madonna Lily, and after the twelfth century it is always used as the symbol of purity, associated with the Virgin. Later, after the fourteenth century, it is very occasionally...

Telomerase

Immortal cancer cells escape telomere loss by switching on a gene that expresses an enzyme called telomerase. This unusual enzyme is a reverse transcriptase that has an RNA template and a catalytic portion. At each round of DNA replication, telomerase adds onto the existing telomeres the nucleotides that would otherwise have been lost, thus maintaining a constant telomere length. In other words, telomerase acts as an immortalizing enzyme. In addition, it has several associated proteins whose roles are still under investigation.

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