Erbb3

Expression Patterns Related to ER Status Tamoxifen is a frequently prescribed drug for treatment and prevention of breast cancer (7,11,90). ER+ tumors may initially respond to tamoxifen, but the development of tamoxifen resistance is an important clinical problem. A number of groups have compared microarray data of breast carcinomas based on ER status. Hilsenbeck et al. (91) compared gene expression arrays of estrogen-stimulated, tamoxifen-sensitive, and resistant MCF-7 cells grown in a nude...

Clinical Manifestations Of Colorectal Cancer

A major goal of global expression analysis is to provide information that supports an enriched system of classification, either alone or in conjunction with clinical and genetic data. To place this effort in perspective, we sketch the clinical behavior of colon cancer and outline the major clinical-pathological classification systems. Adenocarcinoma arises in the epithelial cells of the colon. The tumor consists of a mass of abnormal glands (hence the name, adenocarcinoma) that invades and,...

Constructing Microarrays

Once a clone set has been selected, fabricating microarrays is quite straightforward. The technology is dependent on the use of a robotic device to deposit DNA (typically a PCR product) from each clone on a solid support, usually a glass microscope slide (9). As an alternative to glass, microarrays can also be printed on nylon membranes for use in radioactive detection systems rather than the fluorescence-based detection used for glass microarrays. Detailed protocols for cDNA spotting are...

Background

These account for approx 20 of all primary tumors of bone, with the majority arising in the second decade, the period of maximal skeletal growth (1). While most of these neoplasms arise sporadically, familial cases have been reported, and there is an increased incidence of osteogenic sarcoma in patients with Li-Fraumeni, Bloom, or Rothmund-Thomson Syndromes, and in patients diagnosed with retinoblastoma (211). There is also an increased incidence of osteosarcoma in patients who have undergone...

References

The Chipping Forecast. 1999 Special Supplement. Nat. Genet. 21, 1. 2. Cheng, L. and Wong, W. H. 2001 Model-based analysis of oligonucleotide arrays model validation, design issues and standard error application. Genome Biol. RESEARCH 0032.1-0032.11. 3. Tseng, G., Oh, M. K., Rohlin, L., Liao, J. C., and Wong, W. H. 2001 Issues in cDNA microarray analysis quality filtering, channel normalization, models of variation and assessment of gene effects. Nucleic Acids Res. 29, 2549-2557. 4. Hunter,...

Multiclass Classification Classifying Multiple Tumor Types

Multiclass classification problems are inherently more difficult than pairwise comparisons. In this section, we review our efforts to perform multiclass tumor classification 49,50 . We explored the general feasibility of molecular cancer diagnosis of common human tumors solely on the basis of tumor gene expression profiles. We first created a gene expression database containing the expression profiles of 218 tumor samples representing 14 common human cancer classes and devised a multiclass...