The Msr System and Age Related Diseases

It is now established that in age-related diseases such as Alzheimer's disease, Parkinson's disease, Huntington's disease and cataractogenesis, oxidized proteins accumulate 85-87 . Furthermore, methionine oxidation has been observed in neurodegenerative diseases despite high expression of the MsrA protein in the cerebellum and neurons of rat brain 67 . Indeed, Gabbita et al. 88 revealed a decrease in MsrA activity in all brain regions studied in an Alzheimer patient compared with control...

ROSInduced Oscillations of Mitochondrial Transmembrane Potential in Cultured Rat Astocytes

Vsevolod BELUSOVa, Linda BAMBRIKb, Aanatoly STARKOVb, Gary FISKUMb, Vladimir SKULACHEVa and Dmitry ZOROVa aA.N. Belozersky Institute of Physico-Chemical Biology Moscow State University, Moscow,119899, Russia b Department of Anesthesiology, University of Maryland School of Medicine, Baltimore, Maryland, USA Abstract. Cell death can be triggered by opening of the mitochondrial permeability transition pore (MPT). A recent study with cardiomyocytes revealed ROS-induced oscillations in mitochondrial...

References

1 Packer, L., The controversial carotenoids. In The Antioxidant Miracle (Packer, L., Colman, C., eds.), John Wiley & Sons Inc., New York (2000) pp. 133-141. 2 Goodwin, T.W., Metabolism, nutrition and function of carotenoids. Annu. Rev. Nutr. 6 (1986) 273-297. 3 Olson, J.A., Biological actions of carotenoids. J. Nutr. 119 (1989) 94-94. 4 Rock, C.L., Carotenoids Biology and treatment. Pharmacol. Ther. 75 (1997) 185-197. 5 Khachik, F., Beecher, G.R., Goli, M.B., Lusby, W.R., Separation,...

H

Malondialdehyde (MDA) and malondialdehyde-thiobarbituric acid (MDA TBA) (1 2) conjugate. 4. Thiobarbituric Acid-Reactive Substances and Malondialdehyde The assay for thiobarbituric acid-reactive substances (TBA-RS) is the most commonly used method to assess LPO. The sample to be analyzed is heated with TBA at low pH value. A pink chromogen, predominantly, but by far not only a TBA-MDA adduct, is formed. It has an absorbance maximum at 532 nm. It is measured at 532 or 535 nm. For...

The Msr System A Key to Longevity

The interesting possibility that the methionine sulfoxide reductases system may play a role in longevity was tested by Stadtman and Hoshi's laboratories using knock-out mice and transgenic Drosophila, respectively 69,82 . Their results suggest that overexpression of MsrA is important in death retardation. Overexpression of MsrA extended the lifespan of Drosophila by up to 70 . In wild-type Drosophila, neurons contain a high ROS production rate and endogenous Msr activity is low, implying that...

Multiplicity of Mitochondrial Functioning

Belozersky Institute of Physico-Chemical Biology, Moscow State University, Russia Abstract. Mitochondria play essential role in the cell functioning. Besides their obvious importance in overall cell energy production, mitochondria support cellular redox homeostasis, produce small signaling molecules, generate steroids, heme and Fe-S cluster proteins, participate in apoptosis, cell proliferation, detoxication and thermogenesis. Production of reactive oxygen species (ROS) may be...

MtNOS Regulation by Mitochondrial Membrane Potential

VALDEZ Laboratory of Free Radical Biology, School of Pharmacy and Biochemistry, University of Buenos Aires. Jumn 956, C1113AAD, Buenos Aires, Argentina Abstract. The state 4-state 3 transition regulates NO release in coupled mitochondria, the rates were 40-50 lower in state 3 than in state 4. The rates of NO production by liver and kidney mitochondria were 1.3 and 0.7 nmol min.mg protein in state 4, and 0.7 and 0.4 nmol min.mg protein in state 3. The state 4-state...

DNA Lesions as Biomarkers of Inflammation and Oxidative Stress A Preliminary Evaluation

Dna Lesion Oxidation

Carine BADOUARD a, Thierry DOUKIa, Patrice FAUREb, Serge HALIMIc, Jean CADETa, Alain FAVIERa and Jean-Luc RAVANAT a' aD partement de Recherche Fondamentale sur la Mati re Condens e, SCIB Laboratoire L sions des Acides Nucl iques , CEA Grenoble F-38054 Grenoble Cedex 9, France bLaboratoire de physiopathologie de l'hypoxie HP2 , UFR de M decine-Pharmacie, Domaine de la Merci, 38706 La Tronche Cedex cService de Diab tologie, d'Endocrinologie et des Maladies de la Nutrition, CHU de Grenoble BP 217...

Lipid Peroxidation Measurements Methodological Approaches and Clinical Importance

ALoges-School of Physical Medicine, Institute of Physical Medicine and Gerontology, Bad Harzburg bResearch Institute for Environmental Medicine at the Heinrich Heine University Duesseldorf, Molecular Aging Research, Duesseldorf, Germany Abstract. Important parameters of lipid peroxidation LPO such as fatty acid loss, formation and accumulation of conjugated dienes, lipid hydroperoxides, short-chain alkanes, F2-isoprostanes, oxysterols, TBA-reactive substances TBA-RS , malon-dialdehyde MDA ,...

Results and Discussion

Figure 1 shows that exposure of E. coli MnSOD to peroxynitrite 0.5 mM caused almost total loss of enzyme activity, an effect similar to that reported previously for hrMnSOD 8 . However, in variance to hrMnSOD, treatment of E. coli MnSOD with authentic NO 1 mM, 2h, 37 C caused ca. 50 loss of enzyme activity Fig. 2 . HPLC analysis of hydrolyzed E. coli MnSOD subjected to either peroxynitrite or NO treatment revealed a peak with the same retention time as authentic 3-NTYR not shown . However, NO...

Role of Oxidative Stress in Aging of the Yeast Saccharomyces cerevisiae

Grzegorz BARTOSZ a,b and Tomasz BILINSKIa aDepartment of Biochemistry and Cell Biology, University of Rzeszow b Department of Molecular Biophysics, University of Lodz Abstract. Yeast is an established model in the studies of aging. Both replicative aging limited capacity of budding and chronologic aging limited capacity to survive in the stationary culture are studied in Saccharomyces cerevisiae Perhaps the most interesting aspect of these studies is the identification of genes affecting the...