In relation to lateral flow tests, protein binding is often incorrectly equated with signal intensity at the test line. More appropriately, protein binding should be equated with the adsorption of protein-based capture reagents to the membrane (2,10,11). Because the capture reagent is the first molecule in the immunocomplex, it must be adsorbed to the membrane irreversibly if the entire immunocomplex is to contribute to the signal. The capture reagent must also retain biological activity with the reactive sites available to the analyte.
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