Although it is not possible to notice the presence of glutaraldehyde in urine as an adulterant by either color or smell of the specimen, concentrations of glu-taraldehyde greater than 2% cause significant decrease in optical absorbance, and its presence can be detected indirectly based on final absorbance rate readings (dA/min) (18). Although the presence of glutaraldehyde as an adulterant can also be detected by GC-MS, Wu et al. (25) described a simple fluorometric method for the detection of glutaraldehyde in urine. When 0.5 mL of urine was heated with 1 mL of 7.7 mmol/L potassium dihydrogen phosphate (pH 3.0) saturated with diethylthiobarbituric acid for 1 h at 96-98°C in a heating block, a yellow-green fluorophore developed if glutaldehyde was present. Shaking the specimen with «-butanol resulted in the transfer of this adduct to the organic layer, which can be viewed under long-wavelength ultraviolet light. Glutaralde-hyde in urine can also be estimated using a fluorometer.
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