Detection of Glutaraldehyde in Adulterated Urine

Although it is not possible to notice the presence of glutaraldehyde in urine as an adulterant by either color or smell of the specimen, concentrations of glu-taraldehyde greater than 2% cause significant decrease in optical absorbance, and its presence can be detected indirectly based on final absorbance rate readings (dA/min) (18). Although the presence of glutaraldehyde as an adulterant can also be detected by GC-MS, Wu et al. (25) described a simple fluorometric method for the detection of glutaraldehyde in urine. When 0.5 mL of urine was heated with 1 mL of 7.7 mmol/L potassium dihydrogen phosphate (pH 3.0) saturated with diethylthiobarbituric acid for 1 h at 96-98°C in a heating block, a yellow-green fluorophore developed if glutaldehyde was present. Shaking the specimen with «-butanol resulted in the transfer of this adduct to the organic layer, which can be viewed under long-wavelength ultraviolet light. Glutaralde-hyde in urine can also be estimated using a fluorometer.

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