Conjugation of proteins to colloidal gold is a process that should not be undertaken lightly. In DOA rapid tests, the sensitivity and reliability of the assay are generally determined by the quality of reagents used in its development. Colloidal gold labels and antibodies form the major detection components. Other physical factors, such as drying and use of surfactants, should also be carefully controlled. Future technologies should aim at improving the consistency and reproducibility of these assays.
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