Abbreviations

Chemically defined medium complementary DNA (sequence) cES-cell medium cynomolgus ES (cells) colony forming unit CFU-erythroid CFU-granulocyte 2'3'-cyclic nucleotide 3'-phosphodiesterase DMSO, acetamide and propylene glycol (freezing medium) dibutyryl-cyclic AMP direct current diethyl pyrocarbonate Dil-Ac-LDL Dil-acetylated-low density lipoprotein DMEM Dulbecco's modified Eagle's medium DPX di-n-butyl phthalate xylene (mountant) ECs endothelial cells (Chapter 11) EDTA ethylenediaminetetraacetic...

Aims and scope of the volume

This book, a practical handbook for researchers using embryonic stem (ES) cells, may be thought of as a cookbook. Some cooks reading a cookbook will just slavishly copy a recipe or two whereas others will learn from it, use it as a sourcebook, and provide their own interpretations resulting in novel dishes. It is hoped that you, the scientists using this volume, will emulate the adventurous chefs and use the protocols and ideas presented here as a stimulus for your own original studies and...

Directed differentiation of cES cells induced by coculture

The wide spectrum of differentiated derivatives obtained from cES cells via teratomas and EBs validates their pluripotency, but the cellular heterogeneity hinders the study of specific phenotypes. Thus we have transposed from the murine to the cynomolgus monkey system methods for haematopoietic and neural lineage-specific differentiation of ES cells, which circumvent the requirements for EB formation, addition of morphogens such as retinoic acid, or positive selection systems. Our methods...